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Millet Seeds and Agar Starch Experiment

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Millet Seeds and Agar Starch Experiment

Question

Some millet seeds were soaked in water for two days. They were then broken into small pieces and placed on the surface of agar containing starch. After two days it was found that the agar no longer contained starch.
(a) Suggest how the test for starch in the agar was carried out.
(b) Explain why there was no starch in the agar after two days.
(c) Why was it necessary to soak the seeds?
(d) Why were the millet seeds broken into small pieces?
(e) State the observation that would be made if the seeds had been soaked in boiling water?
(f) Suggest a control experiment that would have been suitable.

Answer

(a) Iodine solution was poured on the agar
(b) Millet seeds produced amylase; that converts starch to maltose
(c) To activate the enzymes
(d) To increase surface area for exposure of enzymes
(e) Starch would not be digested since the enzymes would be denatured by boiling
(f) Placing millet seeds that have not been soaked in water on the agar or boiled millet seeds on the agar