Question
In an experiment to investigate the action of pepsin on egg albumen, equal amounts of pepsin were added to equal amounts of egg albumen in different test-tubes. The test tubes were placed in water baths at different temperatures. The graph below shows time taken for the enzyme to digest protein in each.
(i) What is the optimum temperature for the enzyme?
(ii) Account for the time taken to digest egg albumen at 45°C
Answer
(i) Optimum temperature 36 ± 1
(ii) At 45°C time taken is more than at 35oC because enzyme /pepsin is being denatured
(ii) At 45°C time taken is more than at 35oC because enzyme /pepsin is being denatured
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