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Laboratory Testing for Starch in a Leaf Sequence

Select Subject  |  Biology


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Laboratory Testing for Starch in a Leaf Sequence

Question

The diagram shown below represents the sequence followed in the laboratory during the testing for starch in the leaf


Laboratory Testing for Starch in a Leaf Sequence

a) What is the importance of the following stages
i) Boiling leaf in water
ii) Boiling leaf in Ethanol
iii) Heating the ethanol indirectly over a water bath

b) Fill in the table below to show the conclusion if the following colours are observed after placing drops of iodine onto the leaf at the end of the experiment


COLOUR OBSERVED CONCLUSION
Blue Black (I)
Brown (II)

Answer

a)

i) Stop metabolic processes / Kill the cells
ii) Remove chlorophyll
iii) To avoid igniting the highly flammable Ethanol

b)

(I) Starch present
(II) Starch absent